Journal: Scientific Reports

Article Title: Linearized texture of three-dimensional extracellular matrix is mandatory for bladder cancer cell invasion

doi: 10.1038/srep36128

Figure Lengend Snippet: Texture of electrospun scaffolds made of PCL fibers deposited on drum collector ( a ) and grid collector ( b ) were investigated by scanning electron microscopy (SEM) and assessed for the ability to support binding and invasion of metastatic T24 cells. The degree of fibril organization (anisotropy index) was estimated on the SEM micrographs by using ImageJ. Presence of T24 cells on electrospun PCL was analyzed 7 days after seeding; histology evaluation was performed on sections obtained from a transverse cut of the scaffold. Metastatic T24 cells were seeded onto MIBC-derived ECM, and 4 days after seeding 3 μm paraffin sections from each sample were used, and hematoxylin-eosin stained sections were histologically evaluated ( c ) one experiment representative of 3 independently performed on MIBC-ECM obtained from 3 different tumors is shown. Cell viability of cells seeded for 7 days on the synthetic scaffold was compared to T24 cells plated on glass-slide (green and red spots show live and dead cells, respectively) ( d ) For panels a,b and d one experiment representative of 3 independently performed is shown.

Article Snippet: T24 cell line (CLS Cell Lines Service GmbH, Eppelheim, Germany) was seeded onto bladder tumor-derived ECM as previously reported .

Techniques: Electron Microscopy, Binding Assay, Derivative Assay, Staining

Journal: International Journal of Molecular Sciences

Article Title: BMP9 Promotes the Proliferation and Migration of Bladder Cancer Cells through Up-Regulating lncRNA UCA1

doi: 10.3390/ijms19041116

Figure Lengend Snippet: The validation of recombinant adenovirus BMP9 and siBMP9. ( A ) The expression of BMP9 in normal bladder mucosa ( n = 126), superficial bladder cancer ( n = 68), and infiltrating bladder cancer ( n = 62) in the Lee Bladder database. p = 0.007; ( B ) The expression levels of BMP9 in T24 and BIU-87 cells were detected by western blot; ( C ) The BMP9 was up-regulated in BIU-87 cells after being transfected with AdBMP9 compared to the control group; ( D ) The BMP9 was down-regulated in T24 cells after being transfected with AdsiBMP9 compared to the control group. Data are shown as mean ± SD. ** p < 0.01.

Article Snippet: Human bladder cancer BIU-87 and T24 cells were obtained from the China Center for Type Culture Collection (CCTCC).

Techniques: Biomarker Discovery, Recombinant, Expressing, Western Blot, Transfection, Control

Journal: International Journal of Molecular Sciences

Article Title: BMP9 Promotes the Proliferation and Migration of Bladder Cancer Cells through Up-Regulating lncRNA UCA1

doi: 10.3390/ijms19041116

Figure Lengend Snippet: BMP9 up-regulated the expression of lncRNA UCA1 in bladder cancer cells. ( A ) Five common lncRNA were screened in BIU-87 cells after transfected with AdBMP9 by RT-PCR; ( B ) The expression of lncRNA UCA1 were verified in BIU-87 cells after transfected with AdBMP9 by RT-PCR; ( C ) The expression of lncRNA UCA1 were tested in T24 cells after being transfected with AdsiBMP9 by RT-PCR; ( D ) The inhibitory effect of siUCA1 were analyzed by RT-PCR in BIU-87 cells after being co-transfected with AdBMP9 and siUCA1. Data are shown as mean ± SD. ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, vs. control groups.

Article Snippet: Human bladder cancer BIU-87 and T24 cells were obtained from the China Center for Type Culture Collection (CCTCC).

Techniques: Expressing, Transfection, Reverse Transcription Polymerase Chain Reaction, Control

Journal: OncoTargets and therapy

Article Title: MicroRNA-132 inhibits migration, invasion and epithelial-mesenchymal transition via TGFβ1/Smad2 signaling pathway in human bladder cancer

doi: 10.2147/OTT.S201731

Figure Lengend Snippet: miR-132 inhibited the migration and invasion of BC cells. ( A ) QRT-PCR analysis of miR-132 expression in T24 cells transfected with miR-132 inhibitor was decreased compared to the mimic control cells. ( B ) miR-132 expression in cells transfected with miR-132 mimic is increased compared to the mimic control cells. ( C ) Wound-healing assays indicated that upregulation of miR-132 inhibits bladder cancer cell migration (×100). ( D ) Transwell experiments showed that the upregulation of miR-132 inhibits the invasion of bladder cancer cell (× 100). Notes: * p <0.05; ** p <0.01.

Article Snippet: Human BC cell lines (T24) and human normal urothelial cell line (SV-HUC-1) were purchased from Solarbio life sciences (Beijing, China).

Techniques: Migration, Quantitative RT-PCR, Expressing, Transfection, Control